Applications of LAMBDA laboratory peristaltic pumps

LAMBDA laboratory peristaltic pumps are utilized in applications in research & development (R&D) as well as industrial laboratories:

Peristaltic pumps for single use system & pharmaceutical research

Important for single use systems in pharmaceutical research are pumps that can be equipped quickly and easily with exchangeable sterile tubing. For precise and safe addition or removal of liquids in processes prone to contamination, LAMBDA laboratory peristaltic pumps are applied in single use systems:

  • Sterile filtration
  • Dosing pumps for cell suspensions (inoculation, harvest pump during perfusion, …)
  • Process validation and drug trails

Applications in fermentations & cell cultures

LAMBDA laboratory peristaltic pumps have been specially developed for long term continuous processes like applied in fermentations and cell cultures, since their features are ideal for aseptic and sterile applications:

  • autoclavable tubing,
  • easy-to-clean device surfaces,
  • compact

During fermentation and cell culturing, the LAMBDA peristaltic pumps are mostly used as remote-controlled pumps for automatic pH-control as well as feed pumps for controlled additions of sterile medium, nutrients, minerals and trace elements.

Many scientific publications mention LAMBDA laboratory peristaltic pumps implemented as feed and/or harvest pumps in fed-batch or continuous fermentation (chemostat, turbidostat or perfusion processes).

Feed flow profiles, exponential feed rate, automatic switch ON/OFF of LAMBDA laboratory peristaltic pumps

As a feed pump, the LAMBDA laboratory peristaltic pumps are used for linear and exponential feed profiles of C-feed during fed-batch processes.

In technical microbiology, the LAMBDA laboratory peristaltic pumps are implemented

  • with a pre-programmed feed flow profile
    (programmed on the pump or remote controlled)
  • for automatic switch ON and OFF of the feed pump.

Parameter control during fermentations and cell culturing

While remote controlled by the SIAM automation software (www.lambda-instruments.com/siam-industrial-fermentation-software/) during fermentations or cell culturing, the actual values of a growth parameter (e.g. pO2 value) can also be used to control the laboratory pump (e.g. Glucose feeding).

Further applications of laboratory peristaltic pumps in the technical microbiology

Further applications of laboratory peristaltic pumps in the technical microbiology are:

  • Aseptic sampling at specific time intervals
  • Creating immobilized cells
  • Automatic addition of liquid anti-foam agent (controlled antifoam-pump)
  • Process optimization with C-Feed in Fed-batch
  • Addition/injection of sterile liquids during biotransformation
  • Dosing of Media for serial and parallel dilutions (LAMBDA laboratory peristaltic pumps with foot-switch: see details www.lambda-instruments.com/news/detail/using-lambda-dosing-pump-for-quick-reproducible-serial-dilution/)
  • Record the growth kinetics
  • Quantification of the metabolic activity of bacterial cultures

Industry 4.0, GMP & GLP compatible

LAMBDA laboratory pumps are GMP & GLP compatible. Thanks to remote control and data recording software (option PNet PC-Software for LAMBDA pumps: www.lambda-instruments.com/pnet-control-software/), the laboratory peristaltic pumps are also used in Industry 4.0 projects.

pH-stat (automatic addition of acid, base or buffers)

Commonly used acid and base pumps for pH-stat controlled by a laboratory fermenter / bioreactor system are LAMBDA PRECIFLOW peristaltic pumps. LAMBDA laboratory peristaltic pumps with higher flow rate ranges (LAMBDA HiFLOW, MAXIFLOW and MEGAFLOW) are used in industrial pH-stat for example during chemical processes or enzymatic reactions.

Acid/base pumps connected to the laboratory fermenter / bioreactor system

In USP laboratories (upstream processing laboratories), the laboratory fermenter / bioreactor system controls the pH automatically with the help of correction liquids base and/or acid.

Acid and base pumps are connected to the bioreactor for automatic pH-control

For automatic pH-control, the LAMBDA PRECIFLOW peristaltic pumps for base and/or acid are simply plugged to the LAMBDA MINIFOR bioreactor, see www.fermentador-bioreactor.com/360-view/, please.

LAMBDA acid and base pumps are also controlled by third-party laboratory reactors digitally from a computer via RS-interface or by an external pH-stat via analogue remote control.

pH-stat

For enzymatic and chemical reactions, LAMBDA laboratory peristaltic pumps work as base pump and/or acid pump connected to third-party laboratory devices such as Mettler M300 ISM 1 channel multiparameter transmitter for pH-stat.

Liquid chromatography and laboratory peristaltic pumps

Together with LAMBDA OMNICOLL fraction collector or as a stand-alone instrument, LAMBDA laboratory peristaltic pumps are very well suited for chromatography:

LAMBDA laboratory peristaltic pump for gradient elution in column chromatography

Biochemists dispense the mobile phase with the help of a LAMBDA laboratory pump to the chromatography column while collecting fractions with LAMBDA OMNICOLL.

The LAMBDA peristaltic pumps are also used for column saturation with upward pump flow.

 

In biochemistry, the same pump gets also connected to the LAMBDA OMNICOLL fraction collector, which turns it into an autosampler for collecting samples.

LAMBDA OMNICOLL connected to a LAMBDA laboratory peristaltic pump is used as autosampler
www.fractioncollector.info

Further applications of LAMBDA laboratory peristaltic pumps in down-stream processing (DSP), biochemistry, enzymology and chemistry

Further applications of LAMBDA laboratory peristaltic pumps are found in biochemistry, enzymology and chemical laboratories:

  • Inclusion bodies (IBs): Dosing of solubilized protein during refolding processes
  • Gradient generation for the purification of isolated islets
    • Automatic gradient elution with the automatic gradient pumps from LAMBDA and PNet PC software
  • Pouring gradient gels
  • Control of oxidation and reduction potential

In chemistry, LAMBDA laboratory peristaltic pumps work as titration pump for precise addition of titrant to analyte as well as reagent pumps for the controlled addition of diluted reactants during exothermic reactions (thermostat).

Visualization of reaction rates or enzymatic activity by pump activity

When the pump is used in chemical or enzymatic reactions, it is important to know how much of the solution has been added:

By activation of the in-built INTEGRATOR of the LAMBDA laboratory peristaltic pump as reagent pump, chemists visualize the reaction rates during hydrolysis of amides, esters, anhydrides etc.
The same principle is used by biochemists for measuring the enzyme activity of various enzymes (esterases, amylases, lipases, proteases, …)

Applications in food technology laboratories

In food industry and their food technology laboratories, LAMBDA peristaltic pumps are in use

  • as acid and/or base pump for automatic pH control (see pH-stat),
  • as part of the sampling system during solid phase extraction of lead, copper and zinc from food samples,
  • controlled pumping of milk during biofilm studies

 

 

Do you wish further information about LAMBDA peristaltic pumps for your application?