Selection of references from the laboratory: LAMBDA peristaltic pumps

Here, you can access the most recent scientific publications related to LAMBDA laboratory peristaltic pumps. For additional references, please visit www.lambda-instruments.com/peristaltic-pumps/#publications.

«The best references for
laboratory peristaltic pumps come from
their successful applications.»

At LAMBDA Laboratory Instruments, we always appreciate receiving your publications showcasing how LAMBDA peristaltic pumps contribute to your laboratory projects.

2024 — Scientific publications as references for LAMBDA peristaltic pumps

Fermentation (feed & harvest)

Continuous bioreactor operations: The reaction volume was maintained at a constant level using a dip tube and LAMBDA PRECIFLOW peristaltic pumps.

Zwerger, P. (2024). Acetic Acid Bioproduction by Acetobacterium woodii in Formate Medium in Continuous Bioreactors (Doctoral dissertation, Technische Universität Wien).
https://doi.org/10.34726/hss.2024.114566

Keywords: Acetogen, Acetobacterium woodii, continuous bioreactor, sustainable acetic acid bioproduction, adaptive laboratory evolution, microorganism, Wood-Ljungdahl pathway, formate, carbon monoxide, bioprocess engineering


 

Chemistry

The viscous slurry (43.38 g wet-silica mixed into 170 g Hydrobrite oil as solvent) was added through the LAMBDA peristaltic pump to the reactor.

Luo, L., Rix, F. C., Stevens, K. A., Kuo, C. L., Zhang, X., Lovell, J. A., Harlan, C.J., Ye, X., & Berg, B. R. (2024). Improved In-Situ MAO Derived Silica Supported Single-Site Metallocene Catalysts. U.S. Patent Application No. 18/253,867.
https://patents.google.com/patent/US20240092947A1/en (2024 June 17)

Classifications: C08F10/00, C08F4/65916, C08F10/02, C08F10/14


 

Protein refolding

Development and optimization of inclusion body (IB) refolding processes: Using a LAMBDA PRECIFLOW peristaltic pump, solubilized protein was continuously added into the refolding buffer (0.8 L of 150 mM phosphate buffer, 1 mM EDTA, 20 μM Nicotinamide adenine dinucleotide, pH 6.0).

Igwe, C. L., Pauk, J. N., Hartmann, T., & Herwig, C. (2024). Quantitative analytics for protein refolding states. Process Biochemistry, 136, 191-201.
https://doi.org/10.1016/j.procbio.2023.11.022

Keywords: Inclusion body, refolding dynamics, protein state, process analytical technology, signal-to-noise-ratio

Fed-batch dilution: The LAMBDA PRECIFLOW peristaltic pump fed the solubilized protein into the 3.6 L Labfors 5 stirred tank reactor (0.8 L refolding buffer) at a constant feed rate (Process P1 = 5.26 mL/h, P2 = 5.61 ml/h and P3 = 6.51 ml/h).

Pauk, J. N., Igwe, C. L., Herwig, C., & Kager, J. (2024). An all-in-one state-observer for protein refolding reactions using particle filters and delayed measurements. Chemical Engineering Science, 119774.

https://doi.org/10.1016/j.ces.2024.119774

Keywords: Process monitoring, particle filter, protein refolding, state-estimation, mechanistic model.

2023 — Scientific publications as references for LAMBDA peristaltic pumps

Nanofluids

The working fluid was pumped through the system using a LAMBDA PRECIFLOW peristaltic pump at a flow rate of 360 ml/h.

Eriksen, A. B. (2023). An Experimental and Numerical Study of the Performance of Carbon Black Nanofluids in a Direct Absorption Solar Collector. (Master's thesis, The University of Bergen).
https://bora.uib.no/bora-xmlui/bitstream/handle/11250/3073833/AgatheBjelland_MasterThesis.pdf (28. November 2023)

Keywords: nanofluid, direct absorption solar collectors, solar energy


 

Periodontology

LAMBDA peristaltic pumps used as liquid culture medium pumps (30 ml/h) in an dynamic in vitro biofilm model under anaerobic conditions

Alonso-Español, A., Bravo, E., Ribeiro-Vidal, H., Virto, L., Herrera, D., Alonso, B. & Sanz, M. (2023). The Antimicrobial Activity of Curcumin and Xanthohumol on Bacterial Biofilms Developed over Dental Implant Surfaces. Int. J. Mol. Sci. 2023, 24, 2335.
https://doi.org/10.3390/ijms24032335

Keywords: curcumin; xanthohumol; peri-implantitis; oral biofilms; scanning electron microscopy; confocal laser microscopy; polymerase chain reaction; in vitro; antibacterial; antibiofilm


 

Fermentation
(pH control)

LAMBDA PRECIFLOW peristaltic pumps: During the batch fermentation (Bacillus licheniformis, 2 litres working volume) in LAMBDA MINIFOR 7L, two pumps automatically adjusted the pH value 6.5 by adding 20% NaOH (w/v) and 1 N HCl (v/v).

Dumitru, M. & Ciurescu, G. (2023). Optimization of the fermentation conditions and survival of Bacillus licheniformis as freeze-dried powder for animal probiotic applications. Scientific Papers. Series D. Animal Science. Vol. LXVI, No. 2, 2023; ISSN 2285-5750; ISSN CD-ROM 2285-5769; ISSN Online 2393-2260; ISSN-L 2285-5750.
www.animalsciencejournal.usamv.ro/pdf/2023/issue_2/Art10.pdf (2024 Jan. 02)

Keywords: animal nutrition, Bacillus, bioreactor, freeze-drying, probiotics


 

pH-stat

During the synthesis, the pH was maintained by using the LAMBDA PRECIFLOW peristaltic pump adding 2M NaOH.

Dib, M. A., Gore, E. & Grisel, M. (2023). Intrinsic and rheological properties of hydrophobically modified xanthan synthesized under green conditions. Food Hydrocolloids, Volume 138, 2023, 108461, ISSN 0268-005X.
https://doi.org/10.1016/j.foodhyd.2023.108461

Keywords: Hydrophobically modified xanthan; Green modification; Thermal analysis; Intrinsic and viscometric properties; Rheological behaviour


 

Protein refolding

Protein refolding reaction via fed-batch dilution: Solubilized protein (LDH in buffer) fed in three experiments at constant feed-rates (5.26 ml/h; 5.61 ml/h; 6.51 ml/h) using the peristaltic pump LAMBDA PRECIFLOW into a 3.6 L stirred tank reactor (initial volume: 0.8L).

Pauk, J. N.; Igwe, Ch. L; Herwig, Ch. & all (2023). Full-state monitoring of protein refolding reactions using particle filters and delayed measurements. Authorea. August 19, 2023.
https://doi.org/10.22541/au.169246444.43625502/v1

Keywords: Mechanistic model; particle filter; process monitoring; protein refolding; state-estimation

During the enzyme refolding process, 24 ml cofactor hemin was added over 10 h using a PID-Feed forward control (Lucullus) with a LAMBDA PRECIFLOW peristaltic pump in combination with a Sartorius Entris scale

Humer, D. & Ebner, J. (2023). The Purification of Heme Peroxidases from Escherichia coli Inclusion Bodies (IBs): A Success Story Shown by the Example of Horseradish Peroxidase. In: Kopp, J., Spadiut, O. (eds) Inclusion Bodies. Methods in Molecular Biology, vol 2617. Humana, New York, NY.
https://doi.org/10.1007/978-1-0716-2930-7_16

Keywords: Inclusion bodies, Horseradish peroxidase, Heme peroxidases, Escherichia coli, Refolding, Hydrophobic interaction chromatography, Recombinant, Reversed phase high-performance liquid chromatography


 

Chromatography

The laboratory set-up consisted of a LAMBDA MULTIFLOW peristaltic pump and a vertical dynamic fixed bed adsorption PTFE column (inner diameter 0.6 cm, height of 12.3 cm) connected to an automatic sampling system.

Kakamouka, K., Gavriel, C., Salonikidou, E.D., Giannakoudakis, D.A., Kostoglou, M., Triantafyllidis, K.S. & Deliyanni, E.A. (2022). Dynamic/column tests for dibenzothiophene (DBT) removal using chemically functionalized carbons: Investigation of the influence of physicochemical properties and breakthrough modeling. Colloids and Surfaces A: Physicochemical and Engineering Aspects, Volume 642, 2022, 128597, ISSN 0927-7757,
https://doi.org/10.1016/j.colsurfa.2022.128597

Keywords: deep desulfurization, fuels, dibenzothiophene (DBT) adsorption, activated porous carbon, oxidation of carbon, BAX

Influence of surfactants on vertical transport of fungicide metabolite in soil: 1 ml/min OH-CTL (40 μg/ml hydroxy chlorothalonil in water, 4 ml) on columns, then leaching with water (UPW) pumped continuously 1 ml/min with a LAMBDA PRECIFLOW peristaltic pump.

Báez, M.E., Sarkar, B., Peña,A. Vidal, J., Espinoza, J. & Fuentes, E. (2023). Effect of surfactants on the sorption-desorption, degradation, and transport of chlorothalonil and hydroxy-chlorothalonil in agricultural soils. Environmental Pollution, 2023, 121545, ISSN 0269-7491,
https://doi.org/10.1016/j.envpol.2023.121545

Keywords: leaching, pesticides, soil contamination, sorption and degradation


Hydropedology

The soil monolith got supplied with water via a capillary rainfall simulator connected to a water reservoir and a peristaltic pump LAMBDA MAXIFLOW.

Ehrhardt, A., Berger, K., Filipovic, V., Wöhling, T., Vogel, H. J. & Gerke, H.H. (2022). Tracing lateral subsurface flow in layered soils by undisturbed monolith sampling, targeted laboratory experiments, and model‐based analysis. Vadose Zone Journal, 21(4), e20206.
https://doi.org/10.1002/vzj2.20206

Keywords: soil, monolith, LSF, water saturation, Richards equation, Genuchten-Mualem model

2022 — Scientific publications as references for LAMBDA peristaltic pumps

Fermentation
(feed pump)

LAMBDA MULTIFLOW was used as an external pump of the 2 L fermenter to add the feed solution (600 g/L glucose, 45 g/L KH2PO4, 24 g/L MgSO4, 30 g/L (NH4)2SO4, 1.2 g/L CaCl2 and 150 mL/L of trace metals solution).

Wang, G., Tavares, A., Schmitz, S., França, L., Almeida, H., Cavalheiro, J., Carolas, A., Cavalheiro, J., Ozmerih, S., Blank, L.M., Ferreira, B.S. & Borodina, I. (2022). An integrated yeast‐based process for cis, cis‐muconic acid production. Biotechnology and Bioengineering, 119(2), 376-387.
https://doi.org/10.1002/bit.27992

Keywords: fermentation, metabolic engineering, purification, recovery, Saccharomyces cerevisiae

During chemostat cultivation, a LAMBDA PRECIFLOW peristaltic pump was used for 50 ml/h feed flow regulation.

Zejnilovic, E. (2022). Optimization of process performance and mixotrophic cultivation of Clostridium carboxidivorans for the production of biofuel alcohols. (Doctoral dissertation, TU Vienna).
https://web.archive.org/web/20220805014401id_/https://repositum.tuwien.at/bitstream/20.500.12708/45398/1/Zejnilovic%20Emina%20-%202022%20-%20Optimization%20of%20process%20performance%20and%20mixotrophic...pdf (28. November 2023)

Keywords: renewable energy sources, biofuels, biowaste, residual meterials, waste gases, industrial bioethanol production, acetogenic clostridia, clostridium carboxidivorans

Cultivation in continuous bioreactors with a working volume of 650ml / 1000ml: LAMBDA PRECIFLOW pumped continuously the feed medium and a 1:100 antifoam solution (Polypropylenglycol P2000, Sigma-Aldrich, St. Louis, USA) with a dilution rate of 0.05 1/h and 0.014 1/min, respectively.

Vees, C. A., Herwig, C. & Pflügl, S. (2022). Mixotrophic co-utilization of glucose and carbon monoxide boosts ethanol and butanol productivity of continuous Clostridium carboxidivorans cultures. Bioresource Technology, 353, 127138.
https://doi.org/10.1016/j.biortech.2022.127138

Keywords: Continuous chemostats, Acetogens, Mixotrophic gas fermentation, Carbon and energy efficiency, Metabolic modelling and thermodynamics

The feed-flow rates from 18 mL/h (start fed-batch) to 125 mL/h (end fed-batch) were adjusted with a LAMBDA PRECIFLOW peristaltic pump.

Gundinger, T., Kittler, S., Kubicek, S., Kopp, J., & Spadiut, O. (2022). Recombinant protein production in E. coli using the phoA expression system. Fermentation, 8(4), 181.
https://doi.org/10.3390/fermentation8040181

Keywords: E. coli, phoA promoter, T7lac promoter, pAT, pET, antibody fragment, periplasm, inclusion body

LAMBDA peristaltic pumps as reliable feed- and harvest-pumps during 140 days of continuous fermentation

LAMBDA peristaltic pumps as reliable feed- and harvest-pumps during 140 days of continuous fermentationBrodowski, F., Lezyk, M., Gutowska & Oleskowicz-Popiel, P. (2022). Effect of external acetate on lactate-based carboxylate platform: Shifted lactate overloading limit and hydrogen co-production. Science of The Total Environment, Volume 802, 2022, 149885, ISSN 0048-9697.
doi.org/10.1016/j.scitotenv.2021.149885

Keywords: Chain elongation, Carboxylate platform, Caproate, Hydrogen, Lactate overloading, Acetate

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Cell retention setup
(cell suspension pump)

LAMBDA PRECIFLOW pump continuously circulates the cell suspension as loop flow through the membrane module (to separate cell-free harvest) and remove continuously the bleed flow (to eliminate cells and sustain steady state conditions) in a continuous stirred-tank reactor.

Mainka, T., Herwig, C. & Pflügl, S. (2022). Optimized Operating Conditions for a Biological Treatment Process of Industrial Residual Process Brine Using a Halophilic Mixed Culture. Fermentation, 8(6), 246.
https://doi.org/10.3390/fermentation8060246

Keywords: residual process brine, triple-nutrient limitation, formate and aromatics degradation, continuous bioprocessing, halophilic bioprocess


 

pH-stat

pH-stat: During xanthan succinylation, pH (8.3, 8.5, ..., 9.0) was maintained by an automated control system consisting of a M300 process transmitter (Mettler Toledo), a pH probe (digital ISM pH/ORP sensor InPro 3253i/SG/225; Mettler Toledo) and a PRECILFOW peristaltic pump (LAMBDA Laboratory Instruments) for 2 M NaOH addition.

Abou Dib, M., Hucher, N., Gore, E. & Grisel, M. (2022). Original tools for xanthan hydrophobization in green media: Synthesis and characterization of surface activity. Carbohydrate Polymers, 291, 119548.
https://doi.org/10.1016/j.carbpol.2022.119548

Abou Dib, M. (2022). Synthèse et caractérisation des propriétés de dérivés amphiphiles de xanthane obtenus par voie verte de greffage. Chimie organique. Normandie Université, 2022. Français. NNT: 2022NORMLH24. tel-04240491 (Doctoral dissertation, Normandie Université).
https://theses.hal.science/tel-04240491/ (30. November 2023)

Keywords: Xanthan; Green chemical modification, Alkenyl succinic anhydrides, 1H NMR spectroscopy, Surface active derivatives


 

Chromatography

Fixed-bed column studies: The glass-column (beads bed height = 5 cm, internal diameter = 1.45 cm) was connected to a LAMBDA HiFLOW peristaltic pump at the top end to ensure a 1 mL/min constant flow rate of the five-component heavy metal ion (HMI) solutions containing Zn2+, Pb2+, Cd2+, Ni2+ and Co2+.

Dinu, M.V., Humelnicu, I., Ghiorghita, C.A. & Humelnicu, D. (2022). Aminopolycarboxylic Acids-Functionalized Chitosan-Based Composite Cryogels as Valuable Heavy Metal Ions Sorbents: Fixed-Bed Column Studies and Theoretical Analysis. Gels 2022, 8, 221.
https://doi.org/10.3390/gels8040221

Keywords: aminopolycarboxylic acids; chelating agents; theoretical analysis; fixed-bed column studies; multicomponent heavy metal ions solution

2021 — Scientific publications as references for LAMBDA peristaltic pumps

Chromatography

Experimental Setup in the continuous fixed-bed catalytic system: The liquid flow of the phenol-containing solution was introduced through a LAMBDA PRECIFLOW peristaltic pump through the lower inlet port on the side of the column.

Ferreiro Santiso, C., De Luis Álvarez, A. M., Villota Salazar, N., Lomas Esteban, J. M., Lombraña Alonso, J. I., & Camarero Estela, L. M. (2021). Application of a Combined Adsorption− Ozonation Process for Phenolic Wastewater Treatment in a Continuous Fixed-Bed Reactor.
https://doi.org/10.3390/catal11081014

Keywords: catalytic ozonation, three-phase modelling, fixed-bed reactor, wastewater treatment, phenol, granular activated carbon, Ad/Ox

2020 — Scientific publications as references for LAMBDA peristaltic pumps

Biomedical engineering

LAMBDA MULTIFLOW peristaltic pumps a sheath flow for driving the sample flow into a central core, allowing the particles to pass the uniformly illuminated area of the DFLS system.

Xiao, D., Zang, Z., Sapermsap, N., Wang, Q., Xie, W., Chen, Y. & Li, D.D.U. (2021). Dynamic fluorescence lifetime sensing with CMOS single-photon avalanche diode arrays and deep learning processors. Biomed. Opt. Express 12, 3450-3462 (2021);
https://doi.org/10.1364/BOE.425663

Keywords: DFLS; Dynamic Fluorescence Lifetime Sensing; TCSPC; Time-Correlated Single-Photon Counting; Fast-Moving Particles; Biomedical Science; Fluorescence Lifetimes; Flow Chamber


 

Occlusion formation in the 3D printed middle cerebral artery (MCA) model: The MCA model was connected to a LAMBDA MULTIFLOW, the tubing (ID 3.1 mm) got filled with TBS buffer (pH 7.4) and the thrombus was inserted into the tubing; following the MCA occlusion formation, the flow rate through an arterial branch was 4.5 ml/min.

Vítečková Wünschová, A., Novobilský, A., Hložková, J., Scheer, P., Petroková, H., Jiřík, R., Kulich, P., Bartheldyová, E., Hubatka, F., Jonas, V., Mikulík, R., Malý, P. & Mašek, J. (2020). Thrombus imaging using 3D printed middle cerebral artery model and preclinical imaging techniques: Application to thrombus targeting and thrombolytic studies. Pharmaceutics, 12(12), 1207.
https://doi.org/10.3390/pharmaceutics12121207

Keywords: 3D printing, thrombus imaging, fibrin targeting, thrombolysis, MRI, microCT, fluorescence imaging, rtPA, MCAO


 

Chromatography

Samples (5 mL) were extracted through a PES porous hollow fiber using the LAMBDA PRECIFLOW peristaltic pump in a suspension experiment.

Schroeder, H., Duester, L., Fabricius, A. L., Ecker, D., Breitung, V., & Ternes, T. A. (2020). Sediment water (interface) mobility of metal (loid) s and nutrients under undisturbed conditions and during resuspension. Journal of hazardous materials, 394, 122543.

https://doi.org/10.1016/j.jhazmat.2020.122543

Keywords: Pore water depth profiles, ICP-QQQ-MS, Peeper, mobilization, multi-element


 

The five-component solution (Cu2+, Zn2+, Ni2+, Fe3+ and Cr3+ metal ions) in the dynamic sorption procedure: For a continuous flow of 1.33 ml/min, a LAMBDA HiFLOW peristaltic pump was connected to the top of the small glass column (ID 5 cm, length 16 cm, height of swollen monolith 2.5 cm).

Humelnicu, D., Dragan, E. S., Ignat, M., & Dinu, M. V. (2020). A comparative study on Cu2+, Zn2+, Ni2+, Fe3+, and Cr3+ metal ions removal from industrial wastewaters by chitosan-based composite cryogels. Molecules, 25(11), 2664.
https://doi.org/10.3390/molecules25112664

Keywords: Chitosan, zeolite, cryogel sorbents, dynamic study, metal ions removak

 

2019 — Scientific publications as references for LAMBDA peristaltic pumps

Crystalisation

The solution was collected in a new petri dish each minute, resulting in a total of eight samples. A LAMBDA PRECIFLOW peristaltic pump in combination with transparent silicon tubing (inside ⌀ = 3 mm) was used to create a flow system.

Steendam, R. R., & Frawley, P. J. (2019). Secondary nucleation of sodium chlorate: the role of initial breeding. Crystal Growth & Design, 19(6), 3453-3460.
doi.org/10.1021/acs.cgd.9b00317
Keywords
: Crystallization processes; Sodium chlorate; Water; Secondary nucleation; Chiral solid; Crystals; Fluid shear; Continuous shear-included secondary nucleation; Stationary seed chrystals; Solubility; Laser; Metastable zone; Stationary seed crystals; Seed crystal; surface


 

Fermentation
(feed pump)

Production of recombinant cytochrome in a bioreactor: the feed was pumped through a LAMBDA PRECIFLOW peristaltic pump controlled by the process information management system and its addition was monitored gravimetrically.

Hausjell, J., Schendl, D., Weissensteiner, J., Molitor, C., Halbwirth, H., & Spadiut, O. (2020). Recombinant production of a hard‐to‐express membrane‐bound cytochrome P450 in different yeasts—Comparison of physiology and productivity. Yeast, 37(2), 217-226.
doi.org/10.1002/yea.3441
Keywords: Chalcone 3-hydroxylase, Pichia pastoris, Saccharomyces cerevisiae, Yeast, Physiology, Recombinant Protein Production


 

Biochemical Engineering

LAMBDA Peristaltic Pumps were used with third-party bioreactors for precise pumping of liquids

Hofer, A., Kroll, P. & Herwig, C. (2019). Automated sampling and on-line analytics to increase process understanding. Securecell AG, In der Luberzen 29, CH-8902 Urdorf, Switzerland and TU Wien, Gumpendorfer Strasse 1a, A-1060 Wien, Austria.
doi.org/10.13140/RG.2.2.30419.63523

Keywords: Bioprocesses; Automation technology; Liquid handling; Facilitating; Automated sampling; On-line analytics; Process monitoring and control; Sample processing system; Real-time monitoring; Mammalian cell culture process; Biotechnology


 

Biotechnological research
(feed pump)

LAMBDA PRECIFLOW peristaltic pump fits the requirements for substrate feeding in shaking flasks. The pump can convey flow rates between 0.01 and 60 ml/h.

Wagner, S.G., Mähler, C., Polte, I., von Poschinger, J., Löwe, H., Kremling, A, et al. (2019). An automated and parallelised DIY-dosing unit for individual and complex feeding profiles. Construction, validation and applications. PLoS ONE 14(6): e0217268.
doi.org/10.1371/journal.pone.0217268

Keywords: Industrial applications; Scalable and controllable laboratory procedures; Biotechnological research; Process; Growth rates; Production; feeding strategy; Bioprocess development; Up-scaling; Feeding profiles; Shaking flasks; Ecoli HMS174/DE3; pTRA-51hd plasmid; Substrate; Microencapsulation; Pseudomonas putida; his-tagged eGFP; IMAC.

LAMBDA PRECIFLOW laboratory pump supplied feed following a feed-forward controlled exponential feeding strategy for cell growth (14 h, μ = 0.1 h−1) and induced phase (μ = 0.04 h−1) for protein production in a fed-batch fermentation (Escherichia coli, 5 L – 8 L working volume)

Quehenberger, J., Reichenbach, T., Baumann, N., Rettenbacher, L., Divne, C. & Spadiut, O. (2019). Kinetics and Predicted Structure of a Novel Xylose Reductase from Chaetomium thermophilum. Int. J. Mol. Sci. 2019, 20, 185.
https://doi.org/10.3390/ijms20010185

Keywords: xylose reductase; Chaetomium thermophilum; kinetics; structure; homology model; cofactor binding; stability

2018–2015 — Scientific publications as references for LAMBDA peristaltic pumps

food chemistry

In this novel method, namely centrifugeless dispersive liquid-liquid microextraction, is introduced for the efficient extraction of banned Sudan dyes from foodstuff and water samples: A LAMBDA MULTIFLOW peristaltic pump was applied for the phase separation.

Bazregar, M., Rajabi, M., Yamini, Y., Arghavani-Beydokhti, S., & Asghari, A. (2018). Centrifugeless dispersive liquid-liquid microextraction based on salting-out phenomenon followed by high performance liquid chromatography for determination of Sudan dyes in different species. Food chemistry, 244, 1-6.
https://doi.org/10.1016/j.foodchem.2017.10.006

Keywords: microextraction, salting-out, extraction process, extraction solvent, column, phase separation


 

cell retention setup (bleed & harvest withdrawal)

LAMBDA PRECIFLOW peristaltic pumps were used for bleed and harvest withdrawal in the cell retention setup of a bioreactor.

Mahler, N., Tschirren, S., Pflügl, S. & Herwig, CH. (2018). Optimized bioreactor setup for scale-up studies of extreme halophilic cultures. Biochemical Engineering Journal, Volume 130, 2018, Pages 39-46, ISSN 1369-703X.
doi.org/10.1016/j.bej.2017.11.006

Keywords: Continuous bioprocessing; Bubble column; Cell retention; Halophiles; Salt recycling; Haloferax mediterranei; Saline conditions; Bubble column reactor; Stirred tank creactor; Oxygen tranfer rate; Mass transfer; Continuous haliophilic processes.


 

Ecological Engineering

The best closed reactor was selected, and the flow and contact time operation parameters were optimized for two cycles.The flow was controlled by a LAMBDA MULTIFLOW peristaltic pump.

García de Llasera, M.P., León Santiago, M., Loera Flores, E.J., Bernal Toris, D.N., & Covarrubias Herrera, M.R. (2018). Mini-bioreactors with immobilized microalgae for the removal of benzo(a)anthracene and benzo(a)pyrene from water. Ecological Engineering, Volume 121, 2018, Pages 89-98, ISSN 0925-8574.

https://doi.org/10.1016/j.ecoleng.2017.06.059

doi.org/10.1016/j.ecoleng.2017.06.059
Keywords: PAH, Benzo(a)pyrene, Benzo(a)anthracene, Bioreactors, Microalgae, Alginate, Water bioremediation


 

Biosystems Science & Engineering
(parameter controlled pumps)

Turbidostat composed of two LAMBDA PRECIFLOW peristaltic pumps with RS-232 interface for automated optogenetic regulation of protein production in liquid Escherichia coli cultures

Milias-Argeitis, A., Rullan, M., Aoki, S. K., Buchmann, P., & Khammash, M. (2016). Automated optogenetic feedback control for precise and robust regulation of gene expression and cell growth. Nature communications, 7(1), 12546.
https://doi.org/10.1038/ncomms12546

Keywords: Optogenetics, expression systems, industrial microbiology


 

Adaptive feeding strategy with real time signal (Lucullus) controlled feed rate of the LAMBDA PRECIFLOW pump in fed-batch process

Konakovsky, V., Clemens, C., Müller, M. M., Bechmann, J., Berger, M., Schlatter, S., & Herwig, C. (2016). Metabolic control in mammalian fed-batch cell cultures for reduced lactic acid accumulation and improved process robustness. Bioengineering, 3(1), 5.
https://doi.org/10.3390/bioengineering3010005

Keywords: CHO cell culture, scale-down, fed batch, automation, Lactic acid control, pH, metabolic control, MVDA, uncertainty, online analyzer


 

Chemistry

LAMBDA MULTIFLOW peristaltic pump to evaluate the extraction of lead(II), chromium(III) and copper(II) on a novel adsorbent

Barfi, B., Rajabi, M., Zadeh, M.M. & al. (2015). Extraction of ultra-traces of lead, chromium and copper using ruthenium nanoparticles loaded on activated carbon and modified with N,N-bis-(α-methylsalicylidene)-2,2-dimethylpropane-1,3-diamine. Microchim Acta 182, 1187–1196 (2015).
https://doi.org/10.1007/s00604-014-1434-z

Keywords: Ruthenium nanoparticles loaded on activated carbon; N,N-bis-(α-methylsalicylidene)-2,2-dimethylpropane-1,3-diamine; Metals; Plasma; Food; Water


 

Food technology

PTFE column (25 mm x 7.0 mm i.d.) and LAMBDA MULTIFLOW peristaltic pump: Solid phase extraction of lead, copper, and zinc from different food and water samples

Rajabi, M., Barfi, B., Asghari, A. et al. (2015). Hybrid Amine-Functionalized Titania/Silica Nanoparticles for Solid-Phase Extraction of Lead, Copper, and Zinc from Food and Water Samples: Kinetics and Equilibrium Studies. Food Anal. Methods 8, 815–824 (2015).
doi.org/10.1007/s12161-014-9964-x
Keywords: Hybrid amine-functionalized titania/silica nanoparticles; Food; Water; Solid-phase extraction; Kinetic; Thermodynamic

2014 — Scientific publications as references for LAMBDA peristaltic pumps

Solid-phase extraction (SPE)

LAMBDA MULTIFLOW peristaltic pump used to examine the influence of eluent flow rate (1.0–6.0 ml/min) in highly selective solid phase extraction

Rajabi, M., Mohammadi, B., Asghari, A., Barfi, B. & Behzad, M. (2014). Nano-alumina coated with SDS and modified with salicylaldehyde-5-sulfonate for extraction of heavy metals and their determination by anodic stripping voltammetry. Journal of Industrial and Engineering Chemistry, Volume 20, Issue 5, 2014, Pages 3737-3743, ISSN 1226-086X.
doi.org/10.1016/j.jiec.2013.12.073.

Keywords: Solid phase extraction; Nano-alumina; Sodium dodecyl sulfate (SDS); Sodium salicylaldehyde-5-sulfonate (SAS); Differential pulse anodic stripping voltammetry


Fermentation
(feed pump)

Feed flow rate was kept constant by controlling the speed of the LAMBDA PRECIFLOW peristaltic pump in continuous culture of Methanothermobacter marburgensis

Seifert, A.H., Rittmann, S. & Herwig, C. (2014). Analysis of process related factors to increase volumetric productivity and quality of biomethane with Methanothermobacter marburgensis. Applied Energy, Volume 132, 2014, Pages 155-162, ISSN 0306-2619.
doi.org/10.1016/j.apenergy.2014.07.002.
Keywords: Archaea; Biological methanogenesis; Continuous culture; Gas/liquid mass transfer; CO2; H2; CH4; Quantification


 

cell retention setup

Digital LAMBDA PRECIFLOW as feed pump, bleed pump and cell-free harvest pump to maximize the productivity of extreme halophilic archaeon in a bioreactor equipped with an external cell retention system

Lorantfy, B., Ruschitzka, P. & Herwig, C. (2014). Investigation of physiological limits and conditions for robust bioprocessing of an extreme halophilic archaeon using external cell retention system. Biochemical Engineering Journal, Volume 90, 2014, Pages 140-148, ISSN 1369-703X.
doi.org/10.1016/j.bej.2014.06.004.

Keywords: Halophilic Archaea; Microfiltration for cell retention; Integrated processing; Bioprocess design; Bioprocess monitoring; Ultrafiltration


 

Biofuls
(1.0 mol/L NH4Cl pump)

Inflow of M. marburgensis chemostat culture DoE screening was controlled gravimetrically by designated LAMBDA PRECIFLOW pump set-point

Bernacchi, S., Rittmann, S., Seifert, A. H., Krajete, A. & Herwig, C. (2014). Experimental methods for screening parameters influencing the growth to product yield (Y(x/CH4)) of a biological methane production (BMP) process performed with Methanothermobacter marburgensis. AIMS Bioengineering, 2014, 1(2): 72-87.
doi.org/10.3934/bioeng.2014.2.72
Keywords: Design of Experiments; chemostat; dynamic experiments; bioprocess quantification; carbon balance, biological methanogenesis, bioCH4


 

Ecological Engineering

The influent medium pumped using LAMBDA PRECIFLOW pumps to the columns containing Dehalococcoides for PCE bioremediation

Lacroix, E., Brovelli, A., Maillard, J., Rohrbach-Brandt, E., Barry, D.A. & Holliger, C. (2014). Use of silicate minerals for long-term pH control during reductive dechlorination of high tetrachloroethene concentrations in continuous flow-through columns. Science of The Total Environment, Volumes 482–483, 2014, Pages 23-35, ISSN 0048-9697.
doi.org/10.1016/j.scitotenv.2014.02.099.

Keywords: Organohalide respiration; Microbial reductive dechlorination; PCE; Acidification; pH buffer; Silicate minerals

2013 — Scientific publications as references for LAMBDA peristaltic pumps

Photocatalytic reactor

MULTIFLOW pumped wastewater containing dissolved dye through the reactor with immobilized TiO2

Sima, J., & Hasal, P. (2013). Photocatalytic degradation of textile dyes in a TiO 2/UV system. Chemical Engineering Transactions, 32, 79-84.
https://doi.org/10.3303/CET1332014

Keywords: TiO2 immobilization, wastewater remediation, textile dyes, photoreactor


 

Fermentation
(feed pump)

Feed pump: In a continuous mode of culture, medium was fed by LAMBDA PRECIFLOW peristaltic pump operated on controlled set-points for designated dilution rates (D)

Martinez-Porqueras, E., Rittmann, S. & Herwig, C. (2013). Analysis of H2 to CO2 yield and physiological key parameters of Enterobacter aerogenes and Caldicellulosiruptor saccharolyticus. International Journal of Hydrogen Energy, Volume 38, Issue 25, 2013, Pages 10245-10251, ISSN 0360-3199.
https://doi.org/10.1016/j.ijhydene.2013.06.021
Keywords: Glucose; Xylose; Hydrogen productivity; Hydrogen to carbon dioxide yield; Hydrogen to substrate yield

Feed flow rate kept constant by controlling LAMBDA PRECIFLOW pump speed to achieve a medium dilution rae (D) of 0.05 per hour (h-1) in anaerobic bioreactor

Seifert, A.H., Rittmann, S., Bernacchi, S. & Herwig, C. (2013). Method for assessing the impact of emission gasses on physiology and productivity in biological methanogenesis. Bioresource Technology, Volume 136, 2013, Pages 747-751, ISSN 0960-8524.
https://doi.org/10.1016/j.biortech.2013.03.119.
Keywords: Biomethane production; Industrial and biogenic waste gas; Biological methanogenesis; Bioreactor; Thermophilic culture

Continuous controlled inflow of nutrient medium fed to the bioreactor by LAMBDA PRECIFLOW peristaltic pump to obtain the desired dilution rate (D)

Martinez-Porqueras, E., Wechselberger, P. & Herwig, C. (2013). Effect of medium composition on biohydrogen production by the extreme thermophilic bacterium Caldicellulosiruptor saccharolyticus. International Journal of Hydrogen Energy, Volume 38, Issue 27, 2013, Pages 11756-11764, ISSN 0360-3199.
https://doi.org/10.1016/j.ijhydene.2013.06.124.

Keywords: Caldicellulosiruptor saccharolyticus; Defined medium; Complex medium; Xylose; Double nutrient limitation; Hydrogen productivity


 

Environmental Microbiology

LAMBDA HiFLOW peristaltic pump with Tygon R-3603 tubing used to pump the porewater

Yuheng, W., Frutschi, M., Suvorova, E., Phrommavanh, V., Descostes, M., Osman, A. AA., Geipel, G. & Bernier-Latmani, R. (2013). Mobile uranium(IV)-bearing colloids in a mining-impacted wetland. Nat Commun 4, 2942 (2013)
https://doi.org/10.1038/ncomms3942

Keywords: Pore water pump.

2012 — Scientific publications as references for LAMBDA peristaltic pumps

Fermentation
(feed pump)

Feed pump LAMBDA PRECIFLOW for designated medium dilution rates of Methanothermobacter marburgensis grown in continuous cultures

Rittmann, S., Seifert, A. & Herwig, C. (2012). Quantitative analysis of media dilution rate effects on Methanothermobacter marburgensis grown in continuous culture on H2 and CO2. Biomass and Bioenergy, Volume 36, 2012, Pages 293-301, ISSN 0961-9534.
doi.org/10.1016/j.biombioe.2011.10.038.

Keywords: Methanogenesis; Methanothermobacter marburgensis; Dynamic experiment; Continuous culture; Liquid dilution rate


 

Biogeochemistry Research

Ensure column saturation with upward flow by LAMBDA MULTIFLOW pumps

Maya, C. C., Younga, L., Worsfolda, P. J., Heath, S., Bryan, N. D. & Keith-Roacha, M. J. (2012). The effect of EDTA on the groundwater transport of thorium through sand. Water Res. 2012 Oct 1;46(15):4870-82. Epub 2012 Jun 28. PMID: 22796006.
doi.org/10.1016/j.watres.2012.06.012

Keywords:
Complexing agent; Complexation; Colloid; Migration; Modelling; Speciation


 

2011 — Scientific publications as references for LAMBDA peristaltic pumps

Food technology

Controlled pumping of milk into the Teflon chamber (at flow rate of 340 mL/h and 980 mL/h): Study on Staphylococcus epidermidis adherence

Jaglic, Z., Cervinkova, D., Michu, E. et al. (2011). Effect of milk temperature and flow on the adherence of Staphylococcus epidermidis to stainless steel in amounts capable of biofilm formation. Dairy Science & Technol. 91, 361–372 (2011).
doi.org/10.1007/s13594-011-0017-6

Keywords: Food safety; Staphylococcus epidermidis; Hygiene; Dairy; Biofilm; Adherence.


 

Effluent treatment

LAMBDA PRECIFLOW feed pump with flexible polyethylene tube for optimal growth of algae

Legendre, A. & Desmazieres, N. (2011). Device for Cultivating Algae and/or Microorganisms for Treating an Effluent and Biological Frontage. United States Patent Application 20110318819.
https://www.google.com/patents/EP2367926A2 (1. June 2021)

Keywords: Organohalide respiration; Microbial reductive dechlorination; PCE; Acidification; pH; Buffer; Silicate minerals; Bioremediation


 

Biotechnology

LAMBDA PRECIFLOW feed pump with flexible polyethylene tube

Legendre, A. & Desmazieres, N. (2011). Device for Cultivating Algae and/or Microorganisms for Treating an Effluent and Biological Frontage. United States Patent Application 20110318819.
patents.google.com/patent/EP2367926A2 (1. June 2021)

Keywords: Organohalide respiration; Microbial reducing dechlorination; PCE; Acidification; pH; Buffer, Algae cultivation

2010–2007  — Scientific publications as references for LAMBDA peristaltic pumps

Fermentation
(feed pump)

LAMBDA PRECIFLOW peristaltic pump used as feed pump for glycerol during fermentation of E. coli and for producing chitin beads

Lavallaz, G. & Crelier, S. (2010). Purification de GFP avec et sans marqueur d'affinité. Diploma thesis, HES-SO Valais, Sion.
https://doc.rero.ch/record/22518 (03. June 2021)

Keywords: fluorescent proteins; separation and purification of proteins and peptides


 

Sensor Research

Remote control of LAMBDA peristaltic pump via RS-485 interface for sample addition at 0.5 ml/hour

Scarmagnani, S., Walsh, Z., Lopez, F. B., Slater, C., Macka, M., Paull, B. & Diamond, D. (2009). Photoswitchable Stationary Phase Based on Packed Spiropyran Functionalized Silica Microbeads.  e-J. Surf. Sci. Nanotech. Vol. 7 (2009) 649-652.
doi.org/10.1380/ejssnt.2009.649

Keywords: Photoswitchable microspheres; Monolithic frit; Spiropyrans; Stationary phase; Metal ions


 

Clinical Immunology

Two computer-controlled programmable LAMBDA HiFLOW peristaltic pumps for gradient generation in purification of isolated human islets

Friberg, A., Ståhle, M., Brandhorst, H., Korsgren, O. & Brandhorst, D. (2008). Human Islet Separation Utilizing a Closed Automated Purification System. Cell Transplant. 2008;17(12):1305-13.
doi.org/10.3727/096368908787648100

Keywords: Human islet isolation; Islet purification; Density gradient purification; Closed system


 

Capillary electrophoresis

Injection of samples using LAMBDA MULTIFLOW peristaltic pumps

Stjernlöf, A. (2007). Portable capillary electrophoresis system with LED-absorbance photometric and LED-induced fluorescence detection. Thesis for the degree in Master of Science, Analytical Chemistry, performed at Dublin City University 2007.
kau.diva-portal.org/smash/get/diva2:5248/FULLTEXT01.pdf (1. June 2021)

Keywords: Capillary electrophoresis (CE), LED‐absorbance photometric detector (LED‐AP), LED‐induced fluorescence (LED‐IF) detector, limit of detection (LOD)


 

Cultivation of Microorganisms

Programmable dosing pump LAMBDA MULTIFLOW was used as automatic control pump.

Vanags, J., Rychtera, M., Ferzik, S., Vishkins, M. & Viesturs, U. (2007). Oxygen and Temperature Control during the Cultivation of Microorganisms using Substrate Feeding. Engineering in Life Sciences, 2007, Volume 7, Issue 3, pages 247–252.
doi.org/10.1002/elsc.200620184

Keywords: Bacteria, bioreactors, fermentation; process control; substrate feeding; temperature control; oxygen consumption


 

Animal cell perfusion cultures, CHO (feed & harvest pumps)

 

LAMBDA PRECIFLOW feed & harvest pumps for animal cell perfusion culture with spin-filter

Vallez-Chetreanu, F., Fraisse Ferreira, L.G., Rabe, R., von Stockar, U. & Marison, I.W. (2007). An on-line method for the reduction of fouling of spin-filters for animal cell perfusion cultures. Journal of Biotechnology, Volume 130, Issue 3, 2007, Pages 265-273, ISSN 0168-1656.
doi.org/10.1016/j.jbiotec.2007.04.007.

Keywords: Animal cell; Perfusion culture; Spin-filter; Fouling; Vibration; Piezo-electric

LAMBDA PRECIFLOW feed & harvest pumps in perfusion with spin-filter to study the animal cell retention

Vallez-Chetreanu, F. (2006). Characterization of the mechanism of action of spin-filters for animal cell perfusion cultures. PhD diss. NO 3488, École Polytechnique Fédérale de Lausanne, Switzerland.
https://doi.org/10.5075/epfl-thesis-3488
https://infoscience.epfl.ch/record/78660/files/EPFL_TH3488.pdf (1. Juni 2021)

Keywords: Perfusion culture; CHO animal cell; Separation; Spin–filter; Retention; Fouling; Response surface methodology; Filter vibration; Piezoelectric.

2003 and earlier — Scientific publications as references for LAMBDA peristaltic pumps

Laboratory fermentation
(feed pump)

Feed pump LAMBDA PRECIFLOW in fed-batch and continuous cultures of yeast Saccharomyces cerevisiae to maintain a constant dilution rate (D)

Stark, D., Zala, D., Münch, T., Sonnleitner, B., Marison, I.W. & von Stockar, U. (2003). Inhibition aspects of the bioconversion of l-phenylalanine to 2-phenylethanol by Saccharomyces cerevisiae. Enzyme and Microbial Technology, Volume 32, Issue 2, 2003, Pages 212-223, ISSN 0141-0229.
doi.org/10.1016/S0141-0229(02)00237-5.

Keywords: 2-Phenylethanol; l-Phenylalanine; Saccharomyces cerevisiae; Inhibition kinetics; Metabolic uncoupling

 

LAMBDA PRECIFLOW feed pump for the production of 2-phenylethanol (PEA) by in situ product removal (ISPR) method

Stark, D., Kornmann, H., Munch, T., Sonnleitner, B., Marison, I. W. &  von Stockar, U. (2003). Novel Type of In Situ Extraction: Use of Solvent Containing Microcapsules for the Bioconversion of 2-Phenylethanol from L-Phenylalanine by Saccharomyces cerevisiae. Biotechnol Bioeng. 2003 Aug 20; 83(4):376-85.
doi.org/10.1002/bit.10679

Keywords: In-situ product recovery; Microencapsulation of solvent; 2-phenylethanol; Extractive fermentation; Mass transfer; Fermenter; Fed-batch culture

Concentrated nutrient solutions were fed by a LAMBDA Peristaltic Pump for the production of Erythromycin from the strain Saccharopolyspora erythraea in fed-batch.

Potvin, J. & Péringer, P. (1994). Ammonium regulation in Saccharopolyspora erythraea. Part II: Regulatory effects under different nutritional conditions. Biotechnol Lett 16, 69–74 (1994).
doi.org/10.1007/BF01022626
Keywords: Erythromycin; Saccharopolyspora erythraea;  Antibiotic; Fermentation; Growth rate; Feeding; Fed-batch

pH-stat

pH-stat with LAMBDA PRECIFLOW acid and base pumps to maintain constant pH 4 and to estimate the biomass production

Vicente, A., Castrillo, J. I, Teixeira, J. A. & Ugalde, U. (1998). On-line estimation of biomass through pH control analysis in aerobic yeast fermentation systems. Biotechnol Bioeng. 1998 May 20; 58(4):445-50.
https://doi.org/10.1002/(SICI)1097-0290(19980520)58:4%3C445::AID-BIT12%3E3.0.CO;2-A

Keywords: On-line control; pH control; Growth monitoring; Proton titration; Yeast; Aerobic fermentation; Chemostat

pH-stat with LAMBDA PRECIFLOW pump for accurate quantitative determination of net proton production or consumption in chemostat cultures of Candida utilis

Castrillo, J. I., De Miguel, I. & Ugalde, U. O. (1995). Proton Production and Consumption Pathways in Yeast Metabolism. A Chemostat Culture Analysis, Yeast Vol. 11: 1353-1365 (1995)
doi.org/10.1002/yea.320111404

Keywords: Yeast; N2 pathway; Chemostate; proton production; qH+; mmol h−1 per g biomass; Candida utilis; Glucose; Glycerol; Carbon source; Nitrogen assimilation pathway;

Fermentation
pH-stat

pH was maintained at 3.5 (±0.01) by LAMBDA PRECIFLOW peristaltic pump in whey chemostat culture

Castrillo, J.I. & Ugalde, U.O. (1993). Patterns of energy metabolism and growth kinetics of Kluyveromyces marxianus in whey chemostat culture. Appl Microbiol Biotechnol 40, 386–393 (1993).
doi.org/10.1007/BF00170398

Keywords: Biomass; Whey; Chemostat culture, Kluyveromyces marxianus, Aerobic